Development of haploid and diploid mouse parthenogenones: effect of oocyte aging in vivo.

Diploid Mouse Parthenogenones: Effect of Oocyte Aging in Vivo

Mouse oocytes were collected 13.5-29,5hr after hCG injection at 4hr intervals, then activated with 7% ethanol for lmin. The oocytes col!ected 13.5hr after hCG injection did not respond to ethanol activation. High activation rates {90,2-98.3%) were obtained in oocytes activated 17.5-29.5hr after iniection with hCG. Haploid parthenogenones were dominant though the nurnber decreased as the age of ...

Development of parthenogenetic and fertilized mouse embryos in the uterus and in extra-uterine sites.

Mouse eggs were activated with hyaluronidase in vitro and subsequently transferred to the oviduct. In the female reproductive tract they formed morulae and blastocysts which died soon after implantation. Haploid blastocysts were transferred beneath the kidney capsule and here some formed disorganized egg-cylinder structures in a week. Morulae and blastocysts from haploid and diploid parthenogen...

Effect of Β-Mercaptoethanolon Resumption of Meiosis, in Vitro Maturation and Development of Immature Mouse Oocyte Treated With Dehp (Di-(2-Ethylhexy) Phathalae)

Purpose: The aim of this study was evaluated the effect of β -mercaptoethanol on resumption of meiosis, In vivo maturation of immature mouse oocytes treated with DEHP and embryo development. Materials and Methods: NMRI mouse strain (4-6 weeks) were treated daily with 50,µI DEHP Group 1: 121 immature oocytes were matured in MEM-α medium contain FCS 5%, hCG 7.5 IU/ml, rFsH 100mIU/ml, Group 2: 12...

Reproductive Performance of Mouse Oocyte after In Vivo Exposure of The Ovary to Continuous Wave Ultrasound

P-90: The Effect of Nitric Oxide on Mouse Oocyte in Vitro Maturation in Two and Three Dimensional Conditions

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...